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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Double immunolabeling for FOS and <t>AVT</t> in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, <t>vasotocin;</t> pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.
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Image Search Results


Double immunolabeling for FOS and AVT in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, vasotocin; pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.

Journal: Frontiers in Integrative Neuroscience

Article Title: Preoptic Area Activation and Vasotocin Involvement in the Reproductive Behavior of a Weakly Pulse-Type Electric Fish, Brachyhypopomus gauderio

doi: 10.3389/fnint.2019.00037

Figure Lengend Snippet: Double immunolabeling for FOS and AVT in a transverse section of the POA of a social male of Brachyhypopomus gauderio . (A) Immunolabeling for DAPI (blue). (B) Immunolabeling for FOS (red). Inset in (B) : the arrow shows the nuclear label for FOS. (C) Immunolabeling for AVT (green). The arrow indicates an AVT+ cell. (D) Merge of (A–C) . Blank arrow indicates an AVT+ cell. Inset in (D) : the arrow shows a double-immunolabeled cell for FOS and AVT (yellow). (E) PPa section of the POA immunolabeled for AVT (false color green), blank arrows indicate pPOA cells and filled arrows indicate mPOA cells. Arrowheads indicate AVT+ fibers. (F,G) Diagrams of AVT+ cells; pPOA (dots), mPOA (black dots), gPOA (squares) and fibers (lines) in a rostral (F) and caudal (G) transverse sections. (H) PPp section of the POA immunolabeled for AVT (false color green). Blank arrowheads indicate gPOA cells somata and filled arrowheads indicate fibers. *, ventricle all levels; Rt, rostral; AVT, vasotocin; pPOA, parvocells; mPOA, magnocells; gPOA, gigantocells; PPa, nucleus preopticus ventricularis, anterior subdivision; PPp, nucleus preopticus ventricularis, posterior subdivision; AC, anterior commissure; cT, tectal commissure; DC, central division of dorsal forebrain; DD, dorsal division of the dorsal forebrain; Dld, dorsolateral telencephalon, dorsal subdivision; FB, forebrain bundle; Ha, hypothalamus anterioris; OC, optic chiasm; PGl, preglomeriular nucleus, lateral subdivison; PGm, preglomerular nucleus, medial subdivison; nucleus; PPa, nucleus preopticus periventricularis, anterior subdivision; Sc, suprachiasmatic nucleus; TeO, optic tectum; TSd, torus semicircularis, dorsal subdivision; Vp, ventral telencephalon, posterior subdivision. Bar scale: (A–D) : 20 μm; (E) : 20 μm; (F,G) : 500 μm; (H) : 10 μm. Inset in (B) : 5 μm. Inset in (D) : 10 μm.

Article Snippet: Control sections were incubated with the primary antiserum (anti-AVP) pre-absorbed with an excess of AVT (1 μg/ml; Cat. 66-0-09, American Peptide Company Inc., Sunnyvale, CA, USA) and no labeling was present.

Techniques: Immunolabeling